In Spider-Man: Into the Spider-verse Miles, after being bitten by the radioactive spider, met Gwanda. Then why didn't his spider senses work to detect that Gwanda is one of his kind i.e., a spider woman (in the hair stick scene)?
It's most likely that he hadn't developed it at that time. In the scene where he goes to look at the spider, it shows him getting a wave of his spider-sense, which he had likely never seen before that point.
I figured it was because his spider sense only warms him of danger, not allies. Like when Nick fury is able to surprise Spiderman (by lying in wait) in the Far From Home trailer.