When Hobo-Spidey and Miles infiltrate the Alchemax laboratory out in the forest, they inadvertently come face to face with Dr. Octopus, who in this dimension is actually a female version, Dr. Olivia Octavius. In the screenplay it reads:
My friends actually call me Liv.
(then turning) My enemies call me Doc Ock.
When all the Spiders are grouped up at Aunt May's house and attacked by Doc Ock and her cronies, Aunt May says:
Cute place. Real homey.
Oh great, it’s Liv.
So if only friends of Doc Ock call her Liv, and Aunt May calls her Liv here, is it safe to say that Aunt May is a friend of Olivia? It seems somewhat possible, considering she also acts as a pseudo-Alfred for Spiderman, what with themed cars, a myriad of suit colors and choices, and many technological upgrades.