In Chip 'n Dale, are they male or female chipmunks?
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For a long time, there was nothing specific about the two chipmunks to clearly identify their sex within the old cartoons.
From Disney's own site:
So, if the official line is that they are "brothers", then they must, by definition, both be male.
Historically, it wasn't until the show Chip 'n' Dale: Rescue Rangers that they were ever given clothes or fleshed out personalities. The clothes and personalities are generally male, and there are at least two instances where both of the brothers exhibit an attraction towards female characters.
Tammy the squirrel in Adventures in Squirrelsitting
Foxglove the bat in Good Times, Bat Times
From the Disney Comics Wiki about Chip & Dale:
Plus the page lists them as male in their biobox.
And from Comicvine's entry on Dale:
And from Magical Mouse Schoolhouse:
And from the Disneyland* website:
(*© Disney, All Rights Reserved, Disney Parks & Travel)
This all seems to point to male.
Just go to http://en.wikiquote.org/wiki/Chip_'n_Dale_Rescue_Rangers and do control-F (or Command-F on a Mac) to search for the word "he" on the page, you can find various lines where the characters were referred to as "he", like this one from Kiwi's Big Adventure:
And this one from To the Rescue: Part V:
(Dale's line here about Chip being his "friend" suggests the writer probably didn't imagine them as brothers at the time, regardless of what the people writing the websites quoted by phantom42 and Meat Trademark thought)